Congenital absence of epdidymis or vas deferans Anti-sperm antibodies Failure of fertilisation In females, ICSI is helpful in the following cases Resistant zona pellucida Anti-sperm antibodies Failure of fertilisation
Step-1 Collection of Eggs

Initial consultation: This is same as detailed before. Treatment and Management: As far as the female patient is concerned, the procedure is exactly similar as that of IVF. Acquisition of sperm and subsequent laboratory technique is different for ICSI. Sperm can be provided by the husband in the form of semen or acquired through operation.

Step-2 ICSI

Step-3 Confirmation of Conception Individual mature sperm is then picked with a very fine needle through a very elaborate equipment and injected into the egg which is collected in the similar manner as IVF. The rest of the procedure is similar as IVF.

Surgical aspiration of sperms is carried out by a very fine needle attached to a syringe which is pushed into the tube carrying sperm from testis to the penis or a piece of testicular tissue is obtained surgically and processed in the laboratory to release entrapped mature sperms. Step-4 Embryo Transfer